Modification of the degree of 4-O-methylation of secondary wall glucuronoxylan.
Identifieur interne : 002134 ( Main/Exploration ); précédent : 002133; suivant : 002135Modification of the degree of 4-O-methylation of secondary wall glucuronoxylan.
Auteurs : Youxi Yuan [États-Unis] ; Quincy Teng [États-Unis] ; Chanhui Lee [Corée du Sud] ; Ruiqin Zhong [États-Unis] ; Zheng-Hua Ye [États-Unis]Source :
- Plant science : an international journal of experimental plant biology [ 1873-2259 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Arabidopsis.
- génétique : Arabidopsis, Methyltransferases.
- métabolisme : Acide glucuronique, Methyltransferases, Xylanes.
- physiologie : Paroi cellulaire.
- Méthylation.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Methyltransferases.
- chemical , metabolism : Glucuronic Acid, Methyltransferases, Xylans.
- enzymology : Arabidopsis.
- genetics : Arabidopsis.
- physiology : Cell Wall.
- Methylation.
Abstract
Plant secondary walls are the major constituent of plant biomass targeted for second-generation biofuel production. Therefore, a thorough understanding of how secondary walls are constructed is critical for a better utilization of plant biomass for biofuel production. One of the major components in secondary walls is xylan, which is composed of a linear chain of β-1,4-linked xylosyl residues. In Arabidopsis, about 10% of xylosyl residues in xylan are substituted with glucuronic acid (GlcA), of which 60% are methylated at O-4. By contrast, all of the GlcA substituents in Populus xylan are methylated at O-4. It is not known how the degree of GlcA methylation in xylan is controlled. In this report, we demonstrated that simultaneous T-DNA knockout mutations of the three glucuronoxylan methyltransferase (GXM) genes, GXM1, GXM2, and GXM3/GXMT1, which are specifically expressed in secondary wall-forming cells, led to a complete loss of GlcA methylation in xylan in Arabidopsis stems. Overexpression of GXM2 and GXM3 in wild-type Arabidopsis resulted in an up to 5-fold increase in glucuronoxylan methyltransferase activity and as a result, up to 90% of the GlcA side chains in xylan were methylated as opposed to 60% seen in the wild type. The increased degree of GlcA methylation in xylan had no discernable effects on cell wall sugar composition and lignin monomer composition. These results reveal that the activities of GXM1, GXM2 and GXM3 are responsible for all of the GlcA methylation in xylan in Arabidopsis stems and that the degree of GlcA methylation in xylan can be modified by altered expression of GXMs.
DOI: 10.1016/j.plantsci.2014.01.005
PubMed: 24576763
Affiliations:
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Electronic address: zhye@plantbio.uga.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Biology, University of Georgia, Athens, GA 30602</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author></analytic><series><title level="j">Plant science : an international journal of experimental plant biology</title><idno type="eISSN">1873-2259</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (enzymology)</term><term>Arabidopsis (genetics)</term><term>Cell Wall (physiology)</term><term>Glucuronic Acid (metabolism)</term><term>Methylation (MeSH)</term><term>Methyltransferases (genetics)</term><term>Methyltransferases (metabolism)</term><term>Xylans (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide glucuronique (métabolisme)</term><term>Arabidopsis (enzymologie)</term><term>Arabidopsis (génétique)</term><term>Methyltransferases (génétique)</term><term>Methyltransferases (métabolisme)</term><term>Méthylation (MeSH)</term><term>Paroi cellulaire (physiologie)</term><term>Xylanes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Methyltransferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronic Acid</term><term>Methyltransferases</term><term>Xylans</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arabidopsis</term><term>Methyltransferases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide glucuronique</term><term>Methyltransferases</term><term>Xylanes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Paroi cellulaire</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Methylation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Méthylation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plant secondary walls are the major constituent of plant biomass targeted for second-generation biofuel production. Therefore, a thorough understanding of how secondary walls are constructed is critical for a better utilization of plant biomass for biofuel production. One of the major components in secondary walls is xylan, which is composed of a linear chain of β-1,4-linked xylosyl residues. In Arabidopsis, about 10% of xylosyl residues in xylan are substituted with glucuronic acid (GlcA), of which 60% are methylated at O-4. By contrast, all of the GlcA substituents in Populus xylan are methylated at O-4. It is not known how the degree of GlcA methylation in xylan is controlled. In this report, we demonstrated that simultaneous T-DNA knockout mutations of the three glucuronoxylan methyltransferase (GXM) genes, GXM1, GXM2, and GXM3/GXMT1, which are specifically expressed in secondary wall-forming cells, led to a complete loss of GlcA methylation in xylan in Arabidopsis stems. Overexpression of GXM2 and GXM3 in wild-type Arabidopsis resulted in an up to 5-fold increase in glucuronoxylan methyltransferase activity and as a result, up to 90% of the GlcA side chains in xylan were methylated as opposed to 60% seen in the wild type. The increased degree of GlcA methylation in xylan had no discernable effects on cell wall sugar composition and lignin monomer composition. These results reveal that the activities of GXM1, GXM2 and GXM3 are responsible for all of the GlcA methylation in xylan in Arabidopsis stems and that the degree of GlcA methylation in xylan can be modified by altered expression of GXMs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24576763</PMID><DateCompleted><Year>2015</Year><Month>02</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2259</ISSN><JournalIssue CitedMedium="Internet"><Volume>219-220</Volume><PubDate><Year>2014</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Plant science : an international journal of experimental plant biology</Title><ISOAbbreviation>Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Modification of the degree of 4-O-methylation of secondary wall glucuronoxylan.</ArticleTitle><Pagination><MedlinePgn>42-50</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plantsci.2014.01.005</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0168-9452(14)00016-8</ELocationID><Abstract><AbstractText>Plant secondary walls are the major constituent of plant biomass targeted for second-generation biofuel production. Therefore, a thorough understanding of how secondary walls are constructed is critical for a better utilization of plant biomass for biofuel production. One of the major components in secondary walls is xylan, which is composed of a linear chain of β-1,4-linked xylosyl residues. In Arabidopsis, about 10% of xylosyl residues in xylan are substituted with glucuronic acid (GlcA), of which 60% are methylated at O-4. By contrast, all of the GlcA substituents in Populus xylan are methylated at O-4. It is not known how the degree of GlcA methylation in xylan is controlled. In this report, we demonstrated that simultaneous T-DNA knockout mutations of the three glucuronoxylan methyltransferase (GXM) genes, GXM1, GXM2, and GXM3/GXMT1, which are specifically expressed in secondary wall-forming cells, led to a complete loss of GlcA methylation in xylan in Arabidopsis stems. Overexpression of GXM2 and GXM3 in wild-type Arabidopsis resulted in an up to 5-fold increase in glucuronoxylan methyltransferase activity and as a result, up to 90% of the GlcA side chains in xylan were methylated as opposed to 60% seen in the wild type. The increased degree of GlcA methylation in xylan had no discernable effects on cell wall sugar composition and lignin monomer composition. These results reveal that the activities of GXM1, GXM2 and GXM3 are responsible for all of the GlcA methylation in xylan in Arabidopsis stems and that the degree of GlcA methylation in xylan can be modified by altered expression of GXMs.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Youxi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Teng</LastName><ForeName>Quincy</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, GA 30602, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Chanhui</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Plant Biology, University of Georgia, Athens, GA 30602, USA; Department of Plant and Environmental New Resources, Kyung Hee University, Yongin, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhong</LastName><ForeName>Ruiqin</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Zheng-Hua</ForeName><Initials>ZH</Initials><AffiliationInfo><Affiliation>Department of Plant Biology, University of Georgia, Athens, GA 30602, USA. Electronic address: zhye@plantbio.uga.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>01</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>Ireland</Country><MedlineTA>Plant Sci</MedlineTA><NlmUniqueID>9882015</NlmUniqueID><ISSNLinking>0168-9452</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014990">Xylans</NameOfSubstance></Chemical><Chemical><RegistryNumber>37317-38-7</RegistryNumber><NameOfSubstance UI="C038910">glucuronoxylan</NameOfSubstance></Chemical><Chemical><RegistryNumber>8A5D83Q4RW</RegistryNumber><NameOfSubstance UI="D020723">Glucuronic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.-</RegistryNumber><NameOfSubstance UI="D008780">Methyltransferases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020723" MajorTopicYN="N">Glucuronic Acid</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008745" MajorTopicYN="N">Methylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008780" MajorTopicYN="N">Methyltransferases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014990" MajorTopicYN="N">Xylans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arabidopsis</Keyword><Keyword MajorTopicYN="N">Glucuronic acid</Keyword><Keyword MajorTopicYN="N">Methyltransferase</Keyword><Keyword MajorTopicYN="N">Secondary wall</Keyword><Keyword MajorTopicYN="N">Xylan</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>11</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>01</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>01</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>2</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24576763</ArticleId><ArticleId IdType="pii">S0168-9452(14)00016-8</ArticleId><ArticleId IdType="doi">10.1016/j.plantsci.2014.01.005</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Yuan, Youxi" sort="Yuan, Youxi" uniqKey="Yuan Y" first="Youxi" last="Yuan">Youxi Yuan</name></region><name sortKey="Teng, Quincy" sort="Teng, Quincy" uniqKey="Teng Q" first="Quincy" last="Teng">Quincy Teng</name><name sortKey="Ye, Zheng Hua" sort="Ye, Zheng Hua" uniqKey="Ye Z" first="Zheng-Hua" last="Ye">Zheng-Hua Ye</name><name sortKey="Zhong, Ruiqin" sort="Zhong, Ruiqin" uniqKey="Zhong R" first="Ruiqin" last="Zhong">Ruiqin Zhong</name></country><country name="Corée du Sud"><noRegion><name sortKey="Lee, Chanhui" sort="Lee, Chanhui" uniqKey="Lee C" first="Chanhui" last="Lee">Chanhui Lee</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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